Fig. 7
From: 3D-tips: user-friendly mesh barrier pipette tips for 3D-spheroid culture
Fluorescence staining of 3D cell cultures of BxPC-3 and ReNcell VM using the 3D-tip system. a F-actin arrangement of 3D cultures of BxPC-3 cells detected by Alexa Fluor™ 488 Phalloidin (green). b F-actin arrangement of 3D cultures of ReNcell VM detected by Alexa Fluor™ 488 Phalloidin (green). Nuclei were visualized with Hoechst (blue). Scale bar = 50 μm. Images were captured on an Olympus FV3000 confocal microscope