Fig. 8

Long-term effects of the 3D-tip method on cell viability. The culture medium of primary S100β-DsRed OECs spheroids was changed using the 3D-tip method or the 50/50 media exchange (control) method. After 4-day incubation, primary S100β-DsRed OECs (red) migrating out from the spheroids were stained with Hoechst (blue) to visualize nuclei along with the cell-permeant dye Calcein AM (green) to determine cell viability and DRAQ7 dye (pink), which stains dead cells. Scale bar = 1000 μm. n = 2. Images were captured on a Nikon Ti2-E inverted microscope