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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Evaluation of human primary intestinal monolayers for drug metabolizing capabilities

Fig. 1

Overview of two scaffold systems used for metabolic assays. a Schematic of the culture conditions. Proliferative cells (green) were harvested and placed onto the two scaffolds in a growth-factor rich medium (purple, expansion medium). When the monolayer became confluent (blue cells) at day 5, the culture medium was exchanged for a medium without added intestinal growth factors (grey, differentiation medium). By day 10, the cells were differentiated and nonproliferative (red) and suitable for metabolic activity assays and possess a brush border i.e. microvilli (yellow). b Summary of the timing of the various assays performed in the study. c QTAP SRM quantification of the protein concentration (pmol of DME protein/mg of total cell protein) in the monolayer cells cultured on the gradient cross-linked and conventional scaffolds at day 10 of culture or in cells isolated from fresh crypts/villi. Shown is the average and standard deviation of the data (n = 3)

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