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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Evaluation of human primary intestinal monolayers for drug metabolizing capabilities

Fig. 2

Assessment of DME activity in cells cultured on the monolayer platforms. a CYP3A4 activity was measured in the monolayers with and without CYP3A4 induction by prior culture in the presence of rifampicin. Rifampicin-induced cells were also assayed in the presence of the CYP3A4 inhibitor ketoconazole. Activity was reported as relative light units (RLU) per mg of protein formed over the luciferase reaction time (150 min) for panels a and b. b UGT activity was measured in the monolayers with and without UGT induction by prior culture in the presence of chrysin. Chrysin-induced cells were also assayed in the presence of the UGT1A10 inhibitor zafirlukast. c Esterase activity was measured in the monolayers with and without the CES2 inhibitor loperamide. Shown is the average and standard deviation of the data (n = 4)

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