Table 1 Point mutational analysis of the PcaV to Van2 mutant
aTF | Amino acid position* | Uninduced GFP/OD | Max/Min (2.5 mM induction) | ||
|---|---|---|---|---|---|
Vanillin | PCA | Vanillic acid | |||
PcaV | I110; M113; N114 | 34.2 ± 19.3 | 1.6 | 189.9 | 2.7 |
PcaV I110V | V110; M113; N114 | 24.7 ± 6.5 | 1.7 | 304.4 | 8.2 |
PcaV N114A | I110; M113; A114 | 54.8 ± 10 | 2.5 | 18 | 2.2 |
PcaV M113S | I110; S113; N114 | 90.1 ± 21.1 | 3.3 | 5.1 | 4.9 |
PcaV I110V N114A | V110; M113; A114 | 74.8 ± 7.8 | 3.5 | 10.7 | 2.4 |
PcaV I110V M113S | V110; S113; N114 | 561.8 ± 52.9 | 3.3 | 2.4 | 3.9 |
PcaV M113S N114A | I110; S113; A114 | 1830.1 ± 60.1 | 3.1 | 1.1 | 1.6 |
Van2 | V110; S113; A114 | 253.4 ± 0.3 | 5.8 | 1.2 | 2.2 |