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Table 1 Point mutational analysis of the PcaV to Van2 mutant

From: Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes

aTFAmino acid position*Uninduced GFP/ODMax/Min (2.5 mM induction)
VanillinPCAVanillic acid
PcaVI110; M113; N11434.2 ± 19.31.6189.92.7
PcaV I110VV110; M113; N11424.7 ± 6.51.7304.48.2
PcaV N114AI110; M113; A11454.8 ± 102.5182.2
PcaV M113SI110; S113; N11490.1 ± 21.13.35.14.9
PcaV I110V N114AV110; M113; A11474.8 ± 7.83.510.72.4
PcaV I110V M113SV110; S113; N114561.8 ± 52.93.32.43.9
PcaV M113S N114AI110; S113; A1141830.1 ± 60.13.11.11.6
Van2V110; S113; A114253.4 ± 0.35.81.22.2
  1. *Mutated amino acid positions highlighted in bold