Table 2 Further point mutation of Van2 biosensor
aTF | Amino acid position* | Mutated amino acid | Uninduced GFP/OD | Max/Min (2.5 mM induction) | ||
|---|---|---|---|---|---|---|
Vanillin | PCA | Vanillic acid | ||||
Van2 | V110; S113; A114 | X | 199.4 ± 5.9 | 6.25 | 1.20 | 1.70 |
Van3 | A110; S113; A114 | Nonpolar small side chain | 2280.6 ± 131.2 | 1.70 | 0.97 | 1.03 |
Van4 | L110; S113; A114 | Nonpolar medium side chain | 2748.7 ± 114.0 | 1.55 | 0.97 | 1.00 |
Van5 | W110; S113; A114 | Nonpolar bulky side chain | 3339.0 ± 249.3 | 1.40 | 1.03 | 1.03 |
Van6 | V110; W113; A114 | Nonpolar bulky side chain | 3047.5 ± 175.9 | 1.45 | 1.00 | 0.97 |