Fig. 7

Far-UV CD spetra of the wild-type rKcINU1 and its mutants. The purified samples were measured at a protein concentration of 0.2 mg/mL in 20 mM phosphoric acid (pH 4.5). As illustrated, the strong negative peaks around 218 nm in the far-UV CD spectra implied the typical feature of the enzymatic secondary structure rich in β-sheet. The following quantitative analysis with CDpro software proved the altered ratios of secondary structure elements among the mutants and the wild-type