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Table 1 Kinetics parameters of the wild-type rKcINU1 and its mutants

From: Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability

SampleKm (μM)Vmax (μM•min−1•mg− 1)kcat (s− 1)kcat/Km (μM− 1•s− 1)
WT32.05 ± 1.533240.35 ± 77.93 a3269.51102.01
N362Q33.04 ± 4.113274.40 ± 214.243303.87100.00
N370Q32.73 ± 4.991718.90 ± 143.781734.3752.99
N399Q24.89 ± 1.072338.92 ± 50.922359.9794.82
N467Q76.29 ± 16.21167.10 ± 25.47168.602.21
N526Q37.97 ± 3.43023.79 ± 163.243051.0080.35
  1. The value represented an average of three replications. amean ± SD