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Table 1 Kinetics parameters of the wild-type rKcINU1 and its mutants

From: Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability

Sample

Km (μM)

Vmax (μM•min−1•mg− 1)

kcat (s− 1)

kcat/Km (μM− 1•s− 1)

WT

32.05 ± 1.53

3240.35 ± 77.93 a

3269.51

102.01

N362Q

33.04 ± 4.11

3274.40 ± 214.24

3303.87

100.00

N370Q

32.73 ± 4.99

1718.90 ± 143.78

1734.37

52.99

N399Q

24.89 ± 1.07

2338.92 ± 50.92

2359.97

94.82

N467Q

76.29 ± 16.21

167.10 ± 25.47

168.60

2.21

N526Q

37.97 ± 3.4

3023.79 ± 163.24

3051.00

80.35

  1. The value represented an average of three replications. amean ± SD