Sample | Km (μM) | Vmax (μM•min−1•mg− 1) | kcat (s− 1) | kcat/Km (μM− 1•s− 1) |
---|---|---|---|---|
WT | 32.05 ± 1.53 | 3240.35 ± 77.93 a | 3269.51 | 102.01 |
N362Q | 33.04 ± 4.11 | 3274.40 ± 214.24 | 3303.87 | 100.00 |
N370Q | 32.73 ± 4.99 | 1718.90 ± 143.78 | 1734.37 | 52.99 |
N399Q | 24.89 ± 1.07 | 2338.92 ± 50.92 | 2359.97 | 94.82 |
N467Q | 76.29 ± 16.21 | 167.10 ± 25.47 | 168.60 | 2.21 |
N526Q | 37.97 ± 3.4 | 3023.79 ± 163.24 | 3051.00 | 80.35 |