Fig. 1
From: HMEJ-mediated efficient site-specific gene integration in chicken cells
CRISPR/Cas9 induces targeted DNA cleavage in chicken cells. a Co-transfection of U6-gRNA-pHEf1A-Cas9-mKate and EYFP-Truncated plasmids in chicken DF-1 (48 h); b Flow cytometry analysis of EYFP percentage in co-transfected DF-1 cells in (a); c Experimental scheme for targeted DAZL gene knock out in DF-1 cells; d T7 endonuclease I assay of the DAZL gene mutation in chicken DF-1 cells; e Sequencing analysis of targeted mutation in the DAZL gene