Fig. 2
From: HMEJ-mediated efficient site-specific gene integration in chicken cells
Gene integration in chicken somatic cells mediated by the CRISPR/Cas9 system. a Overview of the designs of site-specific gene integration strategies at the chicken ACTB locus; b Flow cytometry analysis of mCherry (gene integrated) cell proportions using the HITI, HDR and HMEJ strategies; c mCherry+ cells were observed using a fluorescence microscope 7 days after co-transfection of Cas9 plasmid and different donor plasmids; d Sequencing analysis of site-specific integration meditated by HITI, HDR and HMEJ at the chicken ACTB locus