Fig. 3
From: HMEJ-mediated efficient site-specific gene integration in chicken cells
HMEJ mediates robust gene integration in chicken PGCs. a and b Plasmid design for gene integration at the DAZL and Pou5f3 loci; c Efficiency of HITI, HDR and HMEJ gene integration at the DAZL and Pou5f3 genes in PGCs; d PGCs bearing stable integration at DAZL and Pou5f3; e PCR of the site-specific gene integration in DAZL-2A-mCherry and Pou5f3-2A-mCherry cell lines; f Sequencing analysis of site-specific gene integration; G and H. The gonad migration of Pou5f3-2A-mCherry (g) and DAZL-2A-mCherry (h) PGCs were observed in 7 days embryos