Fig. 5
From: HMEJ-mediated efficient site-specific gene integration in chicken cells
HMEJ-mediated ovalbumin gene modification in chicken PGCs. a Plasmid design for gene integration at the ovalbumin locus; b Experimental scheme for ovalbumin gene modification in PGCs; c Single cell PCR analysis of gene integration at the ovalbumin gene; d Sequencing analysis of site-specific gene integration at the ovalbumin gene; e Sequential FACS of OVAL modified PGCs (OVA-GS3-EGFP-CMV-mCherry) and their gonad migration in 7 days embryos