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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in Escherichia coli

Fig. 2

(a) Blueprint of CRIMoClo plasmids. Each plasmid has a MoClo-compatible cloning cassette (purple box), flanked by two terminators (rgnB and tL3 depicted in grey) and followed by a selectable resistance marker (orange arrows), a γ conditional-replication origin (grey box), both enclosed between two FRT sites (grey arrows). All CRIMoClo plasmids have one of four different phage attachment sites (green boxes) for the integration in the chromosome by site-specific recombination. (b) Locations of chromosomal attB sites in the annotated genome of E. coli BW25113 (left) and site-specific recombination of CRIMoClo-based plasmid into the attHK022 site (right)

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