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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in Escherichia coli

Fig. 3

Comparison of dynamical response of luciferase activity of the PBAD-lux construct integrated into attHK022, attP21, attϕ80, attλ in forward (a) and reverse (b) orientation, after the addition of indicated concentrations of arabinose at t = 0 h. The results are averaged from at least two independent biological assays and error bars denote standard deviations. The black dashed line represents the instrument detection limit, defined as three times the standard deviation of the luminescence signal registered in a well filled with growth medium, divided by the averaged OD600 values registered in the same well. All signals at or below the instrument detection limit are not considered as significant

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