Fig. 4
From: Super-antibiofilm effect of N2 plasma treated buffer (NPB) against plant pathogenic bacterium
CLSM imaging analysis on biofilms of Pst DC3000 after the NPB treatment. All the images were shown as the merge of green and red, as in Fig. 3. The first columns in A and B showed biofilms without plasma treatment, as the control. NPB in three different concentrations (1/10, 1/5, 1) was used in the treatment shown in the next three columns in A and B, respectively. Images of nine sections along the depth of biofilm (sections 9 through 1, from the top, shown in D) were obtained for three different concentrations, respectively. The images were analyzed by the ZEN 2012 program for B, C, and D. (a) Fluorescence microscopy images (X600) obtained after the LIVE/DEAD Bacterial Viability assay on the biofilm. (b) 3D CLSM images (X630) as Z-stacks of the nine images visualized at three different angles. (c) Total red intensity determined from the 3D data sets. (d) Red intensity profile as the set of intensity values of sections 1 through 9. All the experiments were repeated at least three times, and statistical significance was determined using one-way ANOVA. (Dunnett T3) (*, P < 0.05; **, P < 0.01, compared with control)