Table 2 Comparison of the reported methods used in SELEX rounds
Monitoring methods | Average duration time | Normal reagents required | Average cost in reagents per assay | Instrument and its average cost | Sensitivity | Focus on phase |
|---|---|---|---|---|---|---|
UV quantification | 0.5 h | ~ | ~ | UV quantification instrument 15,000 USD | ng | Eluting phase |
GBDM | 6 to 9 h | Nucleic acid dye | Below 1 USD | The user-defined mini gel cassette 15 USD UV imager 1500 USD | μg or nM | Binding phase |
Gel-shifting | 2 h | Nucleic acid dye | Below 1 USD | Electrophoresis equipment 1800 USD UV imager 1500 USD | μg | Binding phase |
Agarose gel analysis | 0.5 h | PCR reaction reagent; Nucleic acid dye | Below 1.5 USD | Electrophoresis equipment 1800 USD UV imager 1500 USD | ng | Eluting phase |
qPCR | 2 h | qPCR reaction reagent | 25 USD | Real time PCR amplifier 30,000 USD | pg | Eluting phase |
Fluorescence quantification | 1 h | Fluorophore-labeled primer | 25 USD | Fluorescence quantification instrument 70,000 to 150,000 USD | pg | Eluting phase |
Dot blotting | 7 h | Biotin labelled primer; NC membrane; SA-HRP reagents; ECL chemiluminescent solution | 30 USD | Chemiluminescence apparatus 30,000 USD | ng | Binding phase |
EMSA | 7 h | Labeled aptamer (such asγ-32P and biotin); Nylon membrane; Detection reagents | 30 USD | Electrophoresis equipment 1800 USD Imaging apparatus 50,000 USD | μg | Binding phase |
ELONA | 6 h | Biotin-labeled primer; ELISA plates; SA-HRP reagents; TMB solutions | 30 USD | Microplate reader 20,000 USD | ng | Binding phase |
SPR | 6 h | Sensor chip | 90 USD | SPR instrument 150,000 USD | pg | Binding phase |
Gel-elution assay | 7 h | Target-coupled column; PCR reaction reagent | N/A | Electrophoresis equipment 1800 USD UV imager 1500 USD | μg | Eluting phase |