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Table 2 Comparison of the reported methods used in SELEX rounds

From: SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding

Monitoring methodsAverage duration timeNormal reagents requiredAverage cost in reagents per assayInstrument and its average costSensitivityFocus on phase
UV quantification0.5 h~~UV quantification instrument
15,000 USD
ngEluting phase
GBDM6 to 9 hNucleic acid dyeBelow 1 USDThe user-defined mini gel cassette 15 USD
UV imager 1500 USD
μg or nMBinding phase
Gel-shifting2 hNucleic acid dyeBelow 1 USDElectrophoresis equipment 1800 USD
UV imager 1500 USD
μgBinding phase
Agarose gel analysis0.5 hPCR reaction reagent; Nucleic acid dyeBelow 1.5 USDElectrophoresis equipment 1800 USD
UV imager 1500 USD
ngEluting phase
qPCR2 hqPCR reaction reagent25 USDReal time PCR amplifier
30,000 USD
pgEluting phase
Fluorescence quantification1 hFluorophore-labeled primer25 USDFluorescence quantification instrument
70,000 to 150,000 USD
pgEluting phase
Dot blotting7 hBiotin labelled primer; NC membrane; SA-HRP reagents; ECL chemiluminescent solution30 USDChemiluminescence apparatus
30,000 USD
ngBinding phase
EMSA7 hLabeled aptamer (such asγ-32P and biotin); Nylon membrane; Detection reagents30 USDElectrophoresis equipment 1800 USD
Imaging apparatus 50,000 USD
μgBinding phase
ELONA6 hBiotin-labeled primer; ELISA plates; SA-HRP reagents; TMB solutions30 USDMicroplate reader
20,000 USD
ngBinding phase
SPR6 hSensor chip90 USDSPR instrument
150,000 USD
pgBinding phase
Gel-elution assay7 hTarget-coupled column; PCR reaction reagentN/AElectrophoresis equipment 1800 USD
UV imager 1500 USD
μgEluting phase
  1. Notes: The methods ranked by average cost in reagents per assay; ~ means none cost; N/A means not applicable
  2. GBDM gel-based diffusion method, qPCR real-time quantitative PCR, EMSA Electrophoretic mobility shift assay, ELONA enzyme-linked oligonucleotide assay, SPR Surface plasmon resonance