Fig. 2

Dynamic range of the engineered gene circuit (E. coli co-transformed with plasmids pRHA40 and pRHA12). Fluorescence-based surface response with respect to IPTG and aTc (56 induction conditions tested); mean of four biological replicates. a Mean normalized red fluorescence (AND gate). b Mean normalized green fluorescence (XOR gate). 3D grid constructed from experimental data, colored by interpolation. On the right, 2D plots showing mean plus/minus standard error