Fig. 4

Environmental robustness assessment of the engineered gene circuit (E. coli co-transformed with plasmids pRHA40 and pRHA12). Fluorescence monitoring for each induction condition (IPTG, aTc) when the culture medium changes (pH variation, urea addition, Fe³⁺ addition, and temperature variation). a Normalized red fluorescence. b Normalized green fluorescence. Error bars correspond to standard errors over four biological replicates