Table 1 Strains and plasmids used for this study
| Â | Description | Reference |
|---|---|---|
E. coli Strains | ||
 Top 10 | Host for cloning plasmids | WEIDI Ltd. |
 BL21 (DE3) | Host for protein overexpression and purification | WEIDI Ltd. |
Plasmids | ||
 pET28a (+) | Plasmid for protein overexpression | Novagen |
 pET22b (+) | Plasmid for protein overexpression | Novagen |
 pET28a-P | pET28a vector containing P-protein gene (NCBI No. WP_112929453.1) | This study |
 pET28a-T | pET28a vector containing T-protein gene (NCBI No. WP_099356926.1) | This study |
 pET28a-H | pET28a vector containing H-protein gene (NCBI No. WP_001295377.1) | This study |
 pET28a-L | pET28a vector containing L-protein gene (NCBI No. WP_110826218.1) | This study |
 pET22b-SHMT | pET22b vector containing SHMT gene (NCBI No. WP_072756891.1) | This study |
 pRSFduet-1-KHB-KDC-DhaT | pRSFduet-1 vector containing KHG gene (NCBI No. WP_000800512), KDC gene (NCBI No. WP_046124870) and DhaT gene (NCBI No. WP_004900746) | Laboratory stock [15] |