Fig. 4

JAR spheroid attachment to 3D 3-week RL95–2 epithelial endometrial model in alginate scaffolds. Co-cultures were incubated for 24 h, and cryo-sections (10 μm thick) were stained by H&E. Star (*) denotes JAR spheroids, arrows are directed at the epithelial cell-seeded scaffold. Representative JAR spheroid (A) cultured alone and (B) attached to epithelial culture. High magnification of (C) JAR spheroid, (D) epithelial cells within the macroporous alginate scaffold and (E) the interface between the two cell types. (Bar: A-B: 200 μm; C-E: 100 μm, n = 5). F Co-culture of red-labeled JAR spheroid and green-labeled RL95–2 cell constructs within alginate scaffolds, after 24 h incubation. (Bar: a: 20 μm, b: 100 μm)