Fig. 3
From: Development of a novel heterologous β-lactam-specific whole-cell biosensor in Bacillus subtilis
Growth and luminescence signal of the biosensor constructs in response to different antibiotics. a The graphs represent the detection of the antibiotic penicillin G by the four different biosensor constructs (left column: Biosensor 1 and Biosensor 1 in ΔpenP; right column: Biosensor 2 and Biosensor 2 in ΔpenP) as well as the responses of Control 3 (left column, lux operon without promoter) and Control 5 (right column, Biosensor 2 in ΔpenP lacking the blaR1 receptor construct). Growth measured as OD600nm (y-axis) is depicted in the upper row, while luminescence is shown in relative luminescence units normalized over OD600nm (RLU/OD600nm) below. The graphs demonstrate the first 5 h (x-axis) of growth and development of the luminescence signal post induction with the antibiotic penicillin at 1 h (black dotted line). Concentrations used for induction can be extracted from Table 2. For the full dataset see Supplement 1, Figure S5 and Figure S6. b The log2 fold change of all four biosensors in response to all tested β-lactams and the two controls bacitracin and water. The log2 fold change was calculated using the luminescence output of all biosensors at 2 h post induction (grey dotted line in Fig. 3a) in comparison to the time point of induction (black dotted line in Fig. 3a). We set the threshold for true induction at log2 = 2 as indicated by the black dotted line. The legend below serves for both Figure A and B