Fig. 5
From: Development of a novel heterologous β-lactam-specific whole-cell biosensor in Bacillus subtilis
Dose-response curves of Biosensor 2 in ΔpenP (TM5611) for all β-lactams tested. The y-axis displays the luminescence signal intensity in relative luminescence units over OD600. Serial dilutions of each antibiotic were prepared to assess the biosensor response to the following concentration ranges: ampicillin (1·10− 5- 0.5 μg/ml), carbenicillin (1·10− 5 - 0.5 μg/ml), penicillin G (4·10− 6- 0.25 μg/ml), cefoperazone (3·10− 5- 2 μg/ml), cefalexin (4·10− 6 - 0.25 μg/ml), cefoxitin (2·10− 5 - 1 μg/ml), cephalosporin C (4·10− 5 - 2.5 μg/ml), cefotaxime (2·10− 5 - 1 μg/ml), aztreonam (4·10− 4 - 25 μg/ml) meropenem (8.45·10− 7 - 0.05 μg/ml), and bacitracin (3·10− 3 - 200 μg/ml, see Supplement 1, Figure S9). The assay range varies between the compounds due to the respective MICs