From: Development of a novel heterologous β-lactam-specific whole-cell biosensor in Bacillus subtilis
Strain # | Alias | Genotype description | Resistances | Source |
---|---|---|---|---|
W168 | Control 1 | Bacillus subtilis wild type | none | lab stock |
TMB3090 | Control 2 | W168 sacA::pBS3C_Pveg-lux | chloramphenicol | Popp et al. 2017 [27] |
TMB2841 | Control 3 | W168 sacA::pBS3Clux | chloramphenicol | Pinto et al. 2018 [28] |
TMB3667 | – | W168 penP::kanr | kanamycin | This study |
TMB3668 | – | W168 ybxI::ermr | MLS | This study |
TMB3675 | – | W168 penP::kanrybxI::ermr | kanamycin, MLS | This study |
TMB3641 | Biosensor 1 | W168 sacA::pBS3C_PblaR1I-blaR1I_PblaZ-lux | chloramphenicol | This study |
TMB3713 | Biosensor 1 in ΔpenP | W168 penP::kanr; sacA::pBS3C_PblaR1I-blaR1I_PblaZ-lux | kanamycin, chloramphenicol | This study |
TMB5607 | Control 4 | W168 penP::kanr; lacA::pBS2E_Pveg-blaR1; sacA::pBS3C_PblaZ-lux | kanamycin, MLS, chloramphenicol | This study |
TMB5608 | Biosensor 2 | W168 thrC::pBS4S_PlepA-blaI; lacA::pBS2E_Pveg-blaR1; sacA::pBS3C_PblaZ-lux | spectinomycin, MLS, chloramphenicol | This study |
TMB5609 | Control 5 | W168 penP::kanr; thrC::pBS4S_PlepA-blaI; sacA::pBS3C_PblaZ-lux | kanamycin, spectinomycin, chloramphenicol | This study |
TMB5610 | Inducible Biosensor | W168 penP::kanr; thrC::pBS4S_PlepA-blaI; lacA::pBS2E_PxylA-blaR1; sacA::pBS3C_PblaZ-lux | kanamycin, spectinomycin, MLS, chloramphenicol | This study |
TMB5611 | Biosensor 2 in ΔpenP | W168 penP::kanr; thrC::pBS4S_PlepA-blaI; lacA::pBS2E-Pveg-blaR1; sacA::pBS3C_PblaZ-lux | kanamycin, spectinomycin, MLS, chloramphenicol | This study |