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Table 1 B. subtilis strains developed and tested in this study

From: Development of a novel heterologous β-lactam-specific whole-cell biosensor in Bacillus subtilis

Strain #

Alias

Genotype description

Resistances

Source

W168

Control 1

Bacillus subtilis wild type

none

lab stock

TMB3090

Control 2

W168 sacA::pBS3C_Pveg-lux

chloramphenicol

Popp et al. 2017 [27]

TMB2841

Control 3

W168 sacA::pBS3Clux

chloramphenicol

Pinto et al. 2018 [28]

TMB3667

W168 penP::kanr

kanamycin

This study

TMB3668

W168 ybxI::ermr

MLS

This study

TMB3675

W168 penP::kanrybxI::ermr

kanamycin, MLS

This study

TMB3641

Biosensor 1

W168 sacA::pBS3C_PblaR1I-blaR1I_PblaZ-lux

chloramphenicol

This study

TMB3713

Biosensor 1 in ΔpenP

W168 penP::kanr; sacA::pBS3C_PblaR1I-blaR1I_PblaZ-lux

kanamycin, chloramphenicol

This study

TMB5607

Control 4

W168 penP::kanr; lacA::pBS2E_Pveg-blaR1; sacA::pBS3C_PblaZ-lux

kanamycin, MLS, chloramphenicol

This study

TMB5608

Biosensor 2

W168 thrC::pBS4S_PlepA-blaI; lacA::pBS2E_Pveg-blaR1; sacA::pBS3C_PblaZ-lux

spectinomycin, MLS, chloramphenicol

This study

TMB5609

Control 5

W168 penP::kanr; thrC::pBS4S_PlepA-blaI; sacA::pBS3C_PblaZ-lux

kanamycin, spectinomycin, chloramphenicol

This study

TMB5610

Inducible Biosensor

W168 penP::kanr; thrC::pBS4S_PlepA-blaI; lacA::pBS2E_PxylA-blaR1; sacA::pBS3C_PblaZ-lux

kanamycin, spectinomycin, MLS, chloramphenicol

This study

TMB5611

Biosensor 2 in ΔpenP

W168 penP::kanr; thrC::pBS4S_PlepA-blaI; lacA::pBS2E-Pveg-blaR1; sacA::pBS3C_PblaZ-lux

kanamycin, spectinomycin, MLS, chloramphenicol

This study