Table 1 List of Primers
Genes | Accession number | Forward Primer Reverse Primer | Length (bp) | Tm(°C) |
|---|---|---|---|---|
NANOG | NM_024865.4 | 5′ – ATGCCTCACACGGAGACTGT – 3′ 5′ – AAGTGGGTTGTTTGCCTTTG – 3′ | 103 | 61.19 57.31 |
OCT-4 | NM_002701.6 | 5′ – TCGAGAACCGAGTGAGAGG – 3′ 5′ – GAACCACACTCGGACCACA – 3′ | 125 | 58.14 59.56 |
REX-1 | NM_174900.5 | 5′ – TGGGAAAGCGTTCGTTGAGA − 3′ 5′ – CACCCTTCAAAAGTGCACCG – 3′ | 90 | 59.89 59.97 |
Ki67 | NM_001145966.1 | 5′ – TCAGAATGGAAGGAAGTCAACTG – 3′ 5′ – TCACTCTCATCAGGGTCAGAAG – 3’ | 105 | 58.35 58.90 |
PDX-1 | NM_000209.4 | 5’ – AAGCTCACGCGTGGAAAGG – 3′ 5′ – GGCCGTGAGATGTACTTGTTG – 3’ | 145 | 57.89 52.38 |
NGN-3 | NM_020999.3 | 5’ – CGGTAGAAAGGATGACGCCT – 3′ 5′ – GGTCACTTCGTCTTCCGAGG – 3’ | 138 | 59.54 60.11 |
NKX-6.1 | NM_006168.2 | 5’ – TTGGCCTATTCGTTGGGGAT – 3′ 5′ – GTCTCCGAGTCCTGCTTCTTC – 3’ | 125 | 59.08 60.14 |
MAFA | NM_201589.3 | 5’ – GCACATTCTGGAGAGCGAGA – 3′ 5′ – TTCTCCTTGTACAGGTCCCG – 3’ | 102 | 59.83 58.74 |
ISL-1 | NM_002202.2 | 5’ – TCCCTATGTGTTGGTTGCGG - 3′ 5′ – TTGGCGCATTTGATCCCGTA – 3’ | 200 | 60.32 60.39 |
GLUT-2 | NM_000340.1 | 5’ – GGTTTGTAACTTATGCCTAAG – 3′ 5′ – GCCTAGTTATGCATTGCAG – 3’ | 211 | 52.25 54.24 |
INSULIN | NM_000207.2 | 5’ – CCGCAGCCTTTGTGAACCAACA – 3′ 5′ – TTCCACAATGCCACGCTTCTGC – 3’ | 215 | 64.34 64.45 |
GLP-1R | NM_002062.4 | 5’ – TCGCTGTGAAAATGAGGAGGA – 3′ 5′ – TCACTCCCGCTCTGTGTTTG – 3’ | 189 | 59.38 60.25 |
GLUCAGON | NM_002054.4 | 5’ – TTATTTGGAAGGCCAAGCTGC – 3′ 5′ – GTCTGCGGCCAAGTTCTTCA – 3’ | 110 | 59.45 60.88 |
18S | NR_003286.2 | 5’ – GTGATGCCCTTAGATGTCC – 3′ 5′ – CCATCCAATCGGTAGTAGC – 3’ | 233 | 55.04 54.86 |