Fig. 2
Glucose released per well of a standard 96-square-well fed-batch MTP (a + b) and the 96-square-well “high release ++” fed-batch MTP (c – d) into Wilms-MOPS medium. The left Y-axis represents the absolute amount of released glucose per well and the right Y-axis represents the measured glucose concentration in the experiment per well with VL,96 = 1000 μL/well. All data points are the mean values of measurements of three individual wells. Error-bars indicate the respective standard deviation. Except for the investigated property, all other properties were kept constant. Data in a + b were generated in our laboratory and previously published [14]. a + c) Influence of two osmotic concentrations on the total amount of glucose released. Additionally, in one approach ammonium was omitted. b + d) Influence of varying pH-values on the total amount of glucose released. For the pH-range from 6 to 8, 200 mM MOPS buffer was applied, for pH-values of 4.5 and 5, 200 mM MES buffer was applied. General experimental conditions: temperature T = 37 °C, humidity = 80%, shaking frequency n = 970 rpm, shaking diameter d = 3 mm, VL,96 = 1000 μL/well. No initial glucose was provided in the medium. No microorganisms were inoculated, thus only the sterile medium was filled into the wells. To avoid unintended growth of contaminants, 0.2 g/L NaN3 was added to the medium