Fig. 3
Glucose release of a standard 96-square-well fed-batch MTP (a + b) and of a high release ++ fed-batch MTP (c + d) into commonly applied media. The left Y-axis represents the absolute amount of released glucose per well and the right Y-axis represents the measured glucose concentration in the experiment per well with VL,96 = 1000 μL/well. All data points represent the mean values of measurements of three individual wells. Error-bars indicate the respective standard deviation. The percentages reflect the deviation between the three different media per sample time. Data in a + b were generated in our laboratory and previously published [14]. a + c) Influence of the three synthetic media PBS, Syn6-MES and Wilms-MOPS. b + d) Influence of the three complex media LB, YP and TB. General experimental conditions: temperature T = 37 °C, humidity = 80%, shaking frequency n = 970 rpm, shaking diameter d = 3 mm, VL,96 = 1000 μL/well. No initial glucose was provided in the medium. No microorganisms were inoculated, thus only the sterile medium was filled into the wells. To avoid unintended growth of contaminants, 0.2 g/L NaN3 was added to the medium