Table 1 An overview of techniques and commercially available systems that enable fed-batch cultivations in small-scale
Ambr 15 | Micro-Matrix | RoboLector | BioLector Pro | Enpresso | Standard FeedPlate | |
|---|---|---|---|---|---|---|
Feeding technique | Automated pipetting | Micro-injection valve | Automated pipetting | Micro-pumps | Degradation of polysaccharides | Continuous diffusion from polymer matrix |
Main advantage | Flexible feeding profile and substrates | Flexible feeding profile and substrates | Flexible feeding profile and substrates | Flexible feeding profile | Simple, easy and cheap application in all scales. Continuous feed in standard MTP: No new device necessary | Simple, easy and cheap application; Continuous feed in standard MTP; No new device necessary |
Main drawback | Significant investment, bolus feed | Significant investment, one plate per device | Significant investment, bolus feed | Significant investment | Release is strongly influenced by media and environmental properties | Release is influenced by media and environmental properties |
Applicable for MTPs | No | Yes | Yes | Yes | Yes | Yes |
Parallel cultivations (per device) N if the device/technic does not limit the number of parallel cultivations | 1 × 48 | 1 × 24 | 1 × 96 | 1 × 24 | N × 96 | N × 96 |