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Table 1 An overview of techniques and commercially available systems that enable fed-batch cultivations in small-scale

From: Optimized polymer-based glucose release in microtiter plates for small-scale E. coli fed-batch cultivations

  Ambr 15 Micro-Matrix RoboLector BioLector Pro Enpresso Standard FeedPlate
Feeding technique Automated pipetting Micro-injection valve Automated pipetting Micro-pumps Degradation of polysaccharides Continuous diffusion from polymer matrix
Main advantage Flexible feeding profile and substrates Flexible feeding profile and substrates Flexible feeding profile and substrates Flexible feeding profile Simple, easy and cheap application in all scales.
Continuous feed in standard MTP: No new device necessary
Simple, easy and cheap application; Continuous feed in standard MTP; No new device necessary
Main drawback Significant investment, bolus feed Significant investment, one plate per device Significant investment, bolus feed Significant investment Release is strongly influenced by media and environmental properties Release is influenced by media and environmental properties
Applicable for MTPs No Yes Yes Yes Yes Yes
Parallel cultivations (per device)
N if the device/technic does not limit the number of parallel cultivations
1 × 48 1 × 24 1 × 96 1 × 24 N × 96 N × 96