Table 2 Comparison between the standard fed-batch MTP and the fed-batch MTP that was developed in this study
Standard fed-batch MTP | Fed-batch MTP developed in this study | |
|---|---|---|
Matrix components | ||
Molecular weight of the prepolymer | 20,000 g/mol | 10,000 g/mol |
Addition of hydrophilic co-polymer | No | Yes |
Release characteristics | ||
Absolut glucose release | 17.3 mg in 72 h | 65 mg in 72 h |
Maximum OTR during fed-batch phase of E. coli cultivations | 5 mmol/L/h | 28 mmol/L/h |
Media components influencing the glucose release characteristics | Clear influence of pH, osmotic concentration, ammonium content | Almost no influence of media properties |
Initial glucose burst | Almost not detectable | Significant |