a, b, c SEM photograph of MSC-derived keratinocytes attachment on fiber mats after 84 h seeding. d Cellular growth of seeded keratinocyte in PVA, Ch-PVA, Ch-PVA + Silk fibrous mats determined by MTT. e, f, g, h Fluorescence image of the DAPI stained nuclei of differentiated MSCs to keratinocyte after 84 h seeding. a and e Ch-PVA + Silk fibrous mat, (b and f) Ch-PVA fibrous mat, (c and g) PVA fibrous mat and (h) conventional subculture plate (Control). (Vertical bars: StdDv, n = 4, *p < .05, **p < .01, and #: stand for not significant differences).