Fig. 5

Effect of CNTF on marker gene expression in neurogenic differentiation of SHEDs. SHEDs were cultured in neurogenic medium without (control) or with CNTF (15 ng/L) for the indicated time. qRT-PCR was performed to examine the expression of NES (encoding Nestin) (a), MAP 2 (b), TUBB3 (encoding β-tubulin III) (c), and CHAT (encoding ChAT) (d) genes. Data are form 3 independent experiments in triplicate (n = 3). The data for each gene are normalized with the control on the same day. *p < 0.05, ** p < 0.01, ***p < 0.001 as compared with control on the same day. #p < 0.05, ##p < 0.01, ###p < 0.001 as compared with day 1