Table 1 Primers used in qRT-PCR to examine gene expression
Genes | Primer Sequence | |
|---|---|---|
Forward | Reverse | |
TUBB3 | 5′ AACCAGATAGGGGCCAAGTT 3’ | 5′ GGCCTGAATAGGTGTCCAAA 3’ |
MAP 2 | 5′ ACCAACCACTGCCAGACCT 3’ | 5′ GTGGCGGATGTTCTTCAGAG 3’ |
NES | 5′ AGCCCTGACCACTCCAGTTTAG 3′ | 5′ CCCTCTATGGCTGTTTCTTTCTCT 3’ |
CHAT | 5′ ATGGCCATTGACAACCATCTTCTG 3’ | 5, AACAAGGCTCGCTCCCACAGCTTC 3’ |
GAPDH | 5′ GGACCTGACCTGCCGTCTAG 3’ | 5′ GTAGCCCAGGATGCCCTTGA 3’ |