Fig. 2

Binding of CDR-6xH variants to nickel and to Penta-His antibody. (a) Pili in the tight conformation (CDR-6xH variants or wild-type CDR loops) were bound to nickel-coated plates (gray bars) or uncoated plates (black bars) blocked with BSA, then detected using mab824 (n = 3, mean ± standard deviation, *** p ≤ 0.0005, ** p ≤ 0.005, and * p ≤ 0.05). (b) E. coli cells expressing CDR-6xH variants were added to nickel-coated plates (gray bars) or to uncoated plates (black bars) blocked with BSA (n = 3, mean ± standard deviation, *** p ≤ 0.0005, ** p ≤ 0.005, and * p ≤ 0.05). (c) Pili in the tight conformation (CDR-6xH variants or wild-type CDR loops) were immobilized and incubated with BSA (dark gray bars), mab824 as a control (black bars) or Penta-His antibody (light gray bars) (n = 3, mean ± standard deviation)