Fig. 5
From: FimH as a scaffold for regulated molecular recognition
Effect of mannose or mab21 on CDR-6xH variants’ binding to Penta-his antibody or nickel. (a) For CDR1-6xH-L to ligand Penta-His antibody: KD = 114.20 ± 9.26 nM without mannose (open squares) and 27.70 ± 2.84 nM with mannose (filled squares). (b) For CDR1-6xH-T to Penta-His: KD = 34.54 ± 3.47 nM without mannose (open circles) and 12.71 ± 1.46 nM with mannose (filled circles). (c) For CDR2-6xH-T to Penta-His: KD = 39.61 ± 8.93 nM without mannose (open circles) and 116.1 ± 21.5 nM with mannose (filled circles). (d) For CDR2-6xH-T binding to nickel, KD = 1.53 ± 0.12 nM without mab21 (open circles) and 10.24 ± 0.70 nM with mab21 (filled circles). (e) For CDR2-6xH-T to nickel: KD = 1.53 ± 0.12 nM without mannose (open circles) and 3.72 ± 0.27 nM with mannose (filled circles). Binding curves (n = 3, mean ± standard deviation) were fitted using GraphPad Prism 7 software using a 1:1 binding model