Fig. 6From: FimH as a scaffold for regulated molecular recognitionDetachment of ligand from CDR2-6xH-T in ELISA. Bound ligand (Penta-His antibody or nickel) is detected after one hour of dissociation in buffer alone with no trigger (black bars), in buffer with mab21 (dark gray bars), or in buffer with mannose (light gray bars). Nonspecific binding was subtracted from all measurements (n = 3, mean ± standard deviation, ** p ≤ 0.005, and * p ≤ 0.05)Back to article page