Fig. 2

Conceptual design and operation. a Objective of proposed device showing the relative sizing of the 96-well plate and agar block. b Spheroids (red) grown in a 96-well plate (light grey) with liquid medium (blue). c Funnel manifold (green) with attached agar block (dark grey) mounted on a 96-well plate. d Centrifuge process using centripetal acceleration to move spheroids into the agar block. e Populated agar block removed from funnel manifold. f Agar block encapsulated and paraffin embedded after backfilling with liquid agar (light green). g Top and side view of a histological section of the agar block. Note: Not to scale, spheroids enlarged for clarity