Fig. 6From: Heparinized chitosan stabilizes the bioactivity of BMP-2 and potentiates the osteogenic efficacy of demineralized bone matrixOsteogenic efficacy of hydrogel-DBM composites. a ALP (top) at 1 week and alizarin red S (bottom) at 3 weeks staining images of composites. ALP activity and relative mineralization were evaluated at the same day of staining. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to DBM-free group (MeGC-DBM 0%). b Osteogenic gene expression measured by qRT-PCR at day 4 (Runx2 and ALP) and day 21 (OCN), compared to MeGC. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to DBM-free group (MeGC-DBM 0%)Back to article page