Table 1 Primers employed in the real-time RT-PCR analysis

Aggrecan

Forward: 5′-CCATCTCTACACGCTACACCC-3′

Reverse: 5′-TTGTCTCCATAGCAGCCTTCC-3’

60 °C

Type-II collagen

Forward: 5’-CGTCTACCCCAATCCAGCAAA-3′

Reverse: 5′-AGCAGGCGTAGGAAGGTCAT-3’

60 °C

SOX9

Forward: 5’-CACACTCCTCCTCCGGCATGA-3′

Reverse: 5′-GCGGAAGTCGATAGGGGGCT-3’

63 °C

Integrin β₁

Forward: 5’-GCGAGTGTGGTGTCTGTAAGT-3′

Reverse: 5′-CATTCCTGTGTGCATGTGTCTT-3’

60 °C

GAPDH

Forward: 5’-AGCCAAAAGGGTCATCATCTCT-3′

Reverse: 5′-AGTCCTTCCACGATACCAAAGT-3’

60 °C