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Table 3 Bacterial strains and plasmids used in this study

From: An efficient system for intestinal on-site butyrate production using novel microbiome-derived esterases

Strains and plasmids

Relevant characteristic

Source

E. coli strains

 DH10B

pIndigoBAC-5 without inserted gene

Laboratory collection

 BAC library

DH10B, pIndigoBAC-5 harboring BALB/c mouse gut microbiome DNA

[23]

 tEc

wild type strain isolated from CD-1 mouse intestines

[31]

 tbe1

tEc, pBAD24::tbe1

This study

 tbe2

tEc, pBAD24::tbe2

This study

 L. lactis

Type strain KCTC 3619

Laboratory collection

 S. pneumoniae

Type strain ATCC 49619

Laboratory collection

Plasmids

 pIndigoBAC-5

Cpr, library backbone vector

Laboratory collection

 pBAD24

Ampr, cloning vector

Laboratory collection