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Table 4 Primers used in this study

From: An efficient system for intestinal on-site butyrate production using novel microbiome-derived esterases

Gene name

Direction

Primer sequence (5′ -3′)a

tbe1_promoter_XmaI

Forward

ACTGCCCGGGAGGTCTCACGGATTCAGGAA

tbe1_XbaI

Reverse

ACTCTCTAGATCATTGCAGCATCCATTTAA

tbe1_promoter_XbaI

Forward

ACTCTCTAGATTGTAAATAACTCAGATTTGGT

tbe1_protmoter_overlapped

Reverse

AATTTTTTTCATATTTTCCTCCTGGTATTGCG

tbe2_overlapped

Forward

CAGGAGGAAAATATGAAAAAAATTCTAATCCT

tbe2_PstI

Reverse

ACTCCTGCAGTTATTTCTTGAAAAGTTGAG

  1. a Restriction enzyme recognition sequences and overlapped sequences are underlined
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Journal of Biological Engineering

ISSN: 1754-1611