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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Optimized expression of Hfq protein increases Escherichia coli growth

Fig. 1

Constructed hfq variants and their expression levels. a Constructed hfq gene structure and RBS variants. Four to six nucleotides upstream of Shine Dalgarno (SD) sequence and eight nucleotides downstream of SD designed or randomly generated. To avoid prolonged transcription downstream of the hfq gene, a transcription terminator was added to the gene construct. b GFP-fused Hfq expression levels. The green fluorescence intensity emitted from E. coli DH5α with a hfq variant was measured. Three replicates and mean ± SD. When tested with each other by t-test, there were no statistically significant differences only between the expression levels of variants 2 and 3 (p-value < 0.05)

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