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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Signal amplification and optimization of riboswitch-based hybrid inputs by modular and titratable toehold switches

Fig. 2

Optimization of the toehold switch-based modulators with various promoters and IPTG concentration. a Optimization strategy. Three constitutive promoters – the Anderson promoter collection BBa_J23101 (Relative strength: 0.7), BBa_J23100 (Relative strength: 1) and BBa_J23119 (Relative strength: > 1) –were used and different concentrations of IPTG (1, 10, 100, 1000 μM) were added. The relative strength data is from the Anderson promoter collection (http://parts.igem.org/Promoters/Catalog/Anderson). b Fluorescence measurements with or without coenzyme B12 (30 μM) for trN1-swN1 and modified trN1-swN1 strains in which the promoter for the riboswitch was changed to J23101 or J23119. c Fold-change of GFP expression levels for the strains used in (b). Error bars indicate standard deviations from biological triplicate measurements

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