Fig. 3
Characterization of riboswitch circuits engineered with toehold switch-based modulators. a Scheme of the toehold switch-based modulator circuits. Five AND-computing toehold switches (ACTS_TypeII_N1 [trN1 & swN1], ACTS_TypeII_N3 [trN3 & swN3], ACTS_TypeI_N2 [tr1N2 & sw1N2], ACTS_TypeII_N2 [trN2 & swN2], and ACTS_TypeII_N6 [trN6 & swN6]) were used [23]. The names in the square brackets are used in this study for simplicity. b Heatmap for the combinations of trigger-toehold switch pairs with or without coenzyme B12 (30 μM). The heatmap was drawn using the average fold-change from biological triplicate experiments, and the raw data are listed in Table S3. c Fluorescence measurements for switch-only circuits with or without coenzyme B12 (30 μM). The 10 μM IPTG was used for all tests in Figure 3. Error bars indicate standard deviations from biological triplicate measurements