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Table 3 Summary of the results of comparative studies about static and rocking motion cultures

From: Lymphocyte expansion in bioreactors: upgrading adoptive cell therapy

  Expansion yield Purity Functionality changes
Author - Year Static system (vs.) Static folda Reactor fold Culture days Static Reactor
T cells
 Tran [66] - 2007 Bags 247–1340 200–800 14 > 98% CD4+ Markers of cell activation increased. No detectable Treg cells produced. Cytokines are produced normally.
NK cells
 Sutlu [94] - 2010 Bags 530 77 20 31% NK 38% NK; 14% NKT Degranulation and cytotoxic activity are greater in bioreactor cultures.
 Spanholtz [104] - 2011 Bags 759–1770 1435–2657 42 71 ± 9% CD56 + CD3- 92% ± 2% CD56 + CD3- Higher expression of activating receptors in bioreactor cultures
27% degranulation in reactor vs 14–18% in static cultures
 Lapteva [65] - 2014 G-rex No difference 9 Fewer CD3+ T and a higher CD56 + CD3- NK cells in reactor culture Potency is similar (phenotype and in cytotoxicity assays)
 Meng [97] - 2018 Bags No difference 15 Reactor improves the percentage of NK cells There is no significant modulation of the cells’ secretome. Cytotoxicity is significantly higher for bioreactor cultures.
TIL
 Sadeghi [107] - 2011 Bags 72 ± 11 228.8 ± 17.1 14 No difference in CD8+ and CD4+ percentage No difference in Phenotype
 Somerville [64] -2012 Bags 1259 ± 137 1130 ± 127 14 Lower CD8 and higher CD4 in reactor Increased IFN-γ release to cognate peptide in reactor culture Significant phenotype differences
 Donia [108] - 2014 Bags 1433 ± 887 5576 ± 1677 14 > 97% CD3+
  1. aFold = Harvested cells / Seeded cells