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Table 7 Performance of non-comparative cultures in hollow fiber reactors

From: Lymphocyte expansion in bioreactors: upgrading adoptive cell therapy

Author - Year Expansion Purity Functionality changes
Fold Days Media feed [L]
T cell
 Lamers [125] - 1999 41–149 15 NR Predominantly CD3+ and CD8+ Reduction in cytolytic activity at the end of the culture
 Liu [126] - 1999 2 × 105–108 50–70 NR 95–99% CD4 + CD3+ T cells with virtual elimination of CD8+ cells 50–95% of the cells had elevated expression of HLA-DR.; (IL2R)-a chain expression was increased; 40 to 90% of CD25 levels higher than freshly isolated CD4+ T cells
 Nankervis [111] - 2018 117,450 (1st generation) 13 2.2–2.4 90.9–98.8% CD3+ NR
439–557 (2nd generation) 10 10.4–13.9 98.8–99.5% CD3+ NR
 Coeshott [112] - 2019 543–1079 (high seeding) 8 19.9 91.9–94.5% CD3+;
CD4+ expanded preferentially
T-cell products had had low frequencies of cells bearing exhaustion markers
951–1787 (low seeding) 9 13.6 94.2–97.5% CD3+;
CD8+ expanded preferentially
TIL
 Lewko [117, 129] - 1994 17.3 22.3 40 96% T cells based on CD2+ reactivity. Cells produced cytokines normally
PBL
 Pan [120] - 1999 104–187 11–12 NR NR 57% transduction frequency
 Shankar [121] - 1997 ~ 100 10 5.5 NR 1–10% transduction frequency
 Stroncek [122] - 1999 ~ 200 17 NR NR < 2.5% transduction frequency