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Table 7 Performance of non-comparative cultures in hollow fiber reactors

From: Lymphocyte expansion in bioreactors: upgrading adoptive cell therapy

Author - Year

Expansion

Purity

Functionality changes

Fold

Days

Media feed [L]

T cell

 Lamers [125] - 1999

41–149

15

NR

Predominantly CD3+ and CD8+

Reduction in cytolytic activity at the end of the culture

 Liu [126] - 1999

2 × 105–108

50–70

NR

95–99% CD4 + CD3+ T cells with virtual elimination of CD8+ cells

50–95% of the cells had elevated expression of HLA-DR.; (IL2R)-a chain expression was increased; 40 to 90% of CD25 levels higher than freshly isolated CD4+ T cells

 Nankervis [111] - 2018

117,450 (1st generation)

13

2.2–2.4

90.9–98.8% CD3+

NR

439–557 (2nd generation)

10

10.4–13.9

98.8–99.5% CD3+

NR

 Coeshott [112] - 2019

543–1079 (high seeding)

8

19.9

91.9–94.5% CD3+;

CD4+ expanded preferentially

T-cell products had had low frequencies of cells bearing exhaustion markers

951–1787 (low seeding)

9

13.6

94.2–97.5% CD3+;

CD8+ expanded preferentially

TIL

 Lewko [117, 129] - 1994

17.3

22.3

40

96% T cells based on CD2+ reactivity.

Cells produced cytokines normally

PBL

 Pan [120] - 1999

104–187

11–12

NR

NR

57% transduction frequency

 Shankar [121] - 1997

~ 100

10

5.5

NR

1–10% transduction frequency

 Stroncek [122] - 1999

~ 200

17

NR

NR

< 2.5% transduction frequency