Fig. 1

(A) The overall structure of the construct co-expressing the cardiac peptides and GFP under the control of synthetic nirB promoter and primers for three PCR. (B) results of GFP and Cardiac peptide PCR. Lane 1: the DNA size marker, Lane 2: result of GFP PCR, Lane 3: result of cardiac peptides PCR, Lane 4: Negative control (C) Result of SOEing PCR. Presence of a single band at 1500 bp in electrophoresis confirmed correct performance of SOEing PCR. Lane 1: the DNA size marker, Lane 2: result of SOEing PCR Lane 3: Negative control (D) Results of colony PCR, confirming the presence of constructs in recombinant bacteria. Lane 1: The negative control (prepared PCR mixture applying water instead of DNA template), Lane 2: the DNA size marker, Lane 3: the positive colonies comprising the cardiac hormone expressing construct