Table 1 Sets of primer used for amplification of cardiac peptides and GFP constructs, as well as the ones used for SOEing PCR
Sets of primer | Sequences (5′ → 3′) |
|---|---|
Cardiac peptides amplification | Forward: GACGACGACAAGATGGGCGAATTGAAGCTGCCCTT Reverse: TGATTACGCCAAGCTGCCCTT |
GFP amplification | Forward: AAGGGCAGCTTGGCGTAATCAAAGGAGATATACATATGGTGAGCAAGGGCGAGG Reverse: GAGGAGAAGCCCGGTTAGTGGTGGTGGTGGTGGTGCTTGTACAGCTCGTCCATGCCG |
SOEing PCR | Forward: GACGACGACAAGATGGGC Reverse: GAGGAGAAGCCCGGTTAGTG |