Table 1 Sets of primer used for amplification of cardiac peptides and GFP constructs, as well as the ones used for SOEing PCR
| Sets of primer | Sequences (5′ → 3′) |
|---|---|
| Cardiac peptides amplification |
Forward: GACGACGACAAGATGGGCGAATTGAAGCTGCCCTT Reverse: TGATTACGCCAAGCTGCCCTT |
| GFP amplification |
Forward: AAGGGCAGCTTGGCGTAATCAAAGGAGATATACATATGGTGAGCAAGGGCGAGG Reverse: GAGGAGAAGCCCGGTTAGTGGTGGTGGTGGTGGTGCTTGTACAGCTCGTCCATGCCG |
| SOEing PCR |
Forward: GACGACGACAAGATGGGC Reverse: GAGGAGAAGCCCGGTTAGTG |