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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently

Fig. 1

Comparison of phage original T7 and T7-LacI promoter sequences. The sequence of the non-template strand is shown about T7 and T7-Lac promoters. Positions inside the squares are conserved in all 47 natural phage promoter sequences. Positions in boldface type are common among all consensus promoters. Positions that are underlined are variant parts between original T7 and T7-Lac. The binding region (-17 to -5) and initiation region (-4 to + 6) of the T7 promoter are indicated

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Journal of Biological Engineering

ISSN: 1754-1611