Fig. 2From: CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficientlyGene map for targeted Cas9-mediated gene Knock-In. (A) Map of T7-RNAP in BL21(DE3), including LacI, LacO and T7 RNA Polymerase gene. (B) Integration site in BW25113. (C) map of T7-RNAP integration in ideal condition and the location of PCR product (5496 bp) for sequencing Back to article page