Fig. 3From: CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficientlySchematic illustration of DSB induction and homologous recombination. After preparing competent cells, the pTarget-ybhC and Donor DNA which harbored T7-RNAP and homology arms (HRR and HRL) that targeted a chromosomal locus spanning the middle of ybhC gene and the DSB site were electroporated into cells Back to article page