Fig. 5From: CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficientlyThe T7 Expression system worked efficient in BW25113-T7Expression of sYFP under control of T7-LacI promoter with (+) or without (-) IPTG induction in (A)E. coli BW25113, (B)E. coli BL21(DE3) and (C)E. coli BW25113-T7Back to article page